UV-induction of thymine-containing dimers in Saccharomyces cerevisiae.

نویسندگان

  • W W Fäth
  • M Brendel
چکیده

In haploid and diploid S. cerevisiae the dimer yield ratio TT/CT is found to be 1.2/1 and 1.3/1, resp., at the UV (254 nm) unit dose 1 erg/mm2, the share of TT and CT in a UV (254 nm) lethal hit being 0.7 TT and 0.6 CT. A general formulation of the UV lethal hit is given and discussed. The TT+CT yields obtained for S. cerevisiae are compared to those reported for other organisms. It is found that there obviously exists a directly proportional linear correlation between genome size and TT+CT yield for the UV dose range well below the stationary levels of the TT and CT formation kinetics.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

An improved assay of UV-induced thymine-containing dimers in Saccharomyces cerevisiae.

The method for assaying thymine-containing dimers in yeast is based on highly efficient ([3H]-deoxythymidine-5'-monophosphate) DNA-specific labelling and employs ascending thin layer chromatography. It allows satisfactory quantitative analysis down to UV-doses of 500 erg/mm2.

متن کامل

Fate of thymine-containing dimers in the deoxyribonucleic acid of ultravioletirradiated Bacillus subtilis.

The fate of ultraviolet-induced, thymine-containing dimers in the deoxyribonucleic acid (DNA) of Bacillus subtilis was investigated in both the wild type (UV(R)) and an ultraviolet light-sensitive (UV(S)) mutant. During incubation in the dark, dimers were excised from the DNA of the UV(R)B. subtilis, but remained in the DNA of the UV(S) mutant. About 40% of the excised dimers recovered in the w...

متن کامل

Saccharomyces cerevisiae TFS9, a novel isolated yeast capable of high caffeine-tolerant and its application in biodecaffeination approach

There is a great call for using microbial bio-decaffeination approach to remove caffeinefrom caffeinated products and industrial wastes. We aimed in this study to screen strainsof yeasts which exhibit high caffeine tolerance and to investigate the bio-degradation ofcaffeine under growth conditions. Sixteen yeast strains were isolated from the cultivatedtea soils collected from sites of northern...

متن کامل

Detection of thymine dimers in suprabasal and basal cells of chronically UV-B exposed hairless mice.

An immunocytochemical method was developed to study induction and removal of DNA damage in specific cell populations in the epidermis of hairless mice during chronic ultraviolet (UV) exposure. Identification of mouse suprabasal cells was performed with an immunoperoxidase stain. This stain was shown not to affect the fluorescent nuclear stains, used to reveal DNA and DNA damage. In skin cells f...

متن کامل

Green synthesis of silver nanoparticles: Another honor for the yeast model Saccharomyces cerevisiae

Background and Purpose: Microorganism-based synthesis of nanostructures has recently been noted as a green method for the sustainable development of nanotechnology. Nowadays, there have been numerous studies on the emerging resistant pathogenic bacteria and fungal isolates, the probable inability of bacteria and fungi to develop resistance against silver nanoparticles’ (SNPs) antibacte...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Zeitschrift fur Naturforschung. Section C, Biosciences

دوره 30 6  شماره 

صفحات  -

تاریخ انتشار 1975